Coding
Part:BBa_K2066109
Designed by: Kalen Clifton, Christine Gao, Andrew Halleran, Ethan Jones, Likhitha Kolla, Joseph Maniaci, John Marken, John Mitchell, Callan Monette, Adam Reiss Group: iGEM16_William_and_Mary (2016-10-08)
pTet GFP + TetR (weakened)
This part combines a pTet GFP reporter (BBa_K2066023) and a constitutive tetR expression construct (BBa_K2066022) onto the same plasmid, with a modified RBS on the tetR expression construct in order to replicate the effect of having the tetR construct be on a lower-copy plasmid.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1004
Illegal NheI site found at 1027 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 764
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Categories
Parameters
None |